6WO3
Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody U1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.924, 60.038, 135.373 |
| Unit cell angles | 90.00, 100.00, 90.00 |
Refinement procedure
| Resolution | 29.579 - 2.382 |
| R-factor | 0.2313 |
| Rwork | 0.230 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bkc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.617 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.579 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 27079 | 1048 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 90.9 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.930 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% (w/v) PEG 3500, 0.2M di-ammonium hydrogen citrate, pH 5.0 |
