6WNM

The structure of Pf4r from a superinfective isolate of the filamentous phage Pf4 of Pseudomonas aeruginosa PA01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-23
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0055
Spacegroup name	C 2 2 21
Unit cell lengths	38.109, 47.477, 169.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.333 - 2.580
R-factor	0.2131
Rwork	0.211
R-free	0.24240
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.804
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.333	2.690
High resolution limit [Å]	2.580	2.580
Rmerge	0.172	0.834
Rpim		0.331
Number of reflections	5168	607
<I/σ(I)>	16.5
Completeness [%]	100.0	100
Redundancy	13.7	13.7
CC(1/2)	0.997	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	50 mg/ml protein, 37% PEG 3350, 50 mM Tris, 5% glycerol, 50 mM KCl. Crystals soaked with 1 mM mersalyl overnight for phasing.