6WNJ

The Crystal Structure of Apo Domain-Swapped Trimer Q108K:T51D:A28C:I32C of HCRBPII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97625
Spacegroup name	C 2 2 21
Unit cell lengths	87.933, 160.495, 86.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.640 - 2.100
R-factor	0.2069
Rwork	0.205
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rct
RMSD bond length	0.007
RMSD bond angle	0.880
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.053	0.465
Rmeas	0.073	0.570
Number of reflections	35927	3335
<I/σ(I)>	34.8
Completeness [%]	99.3
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	sodium acetate trihydrate, ammonium sulfate