6WNI
Crystal structure of CldA, the first cyclomaltodextrin glucanotransferase with a three-domain ABC distribution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.194990 |
| Spacegroup name | P 32 |
| Unit cell lengths | 66.440, 66.440, 209.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.502 - 1.660 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qho |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.540 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.060 | 0.565 |
| Number of reflections | 121677 | 5577 |
| <I/σ(I)> | 16.3 | 2 |
| Completeness [%] | 99.5 | 92.8 |
| Redundancy | 7.4 | 4 |
| CC(1/2) | 0.999 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.1 | 291 | 0.2 M ammonium citrate dibasic, 20%(w/v) PEG 3350 |
