6WNF

The Crystal Structure of Apo Domain-Swapped Dimer Q108K:K40D:T53A:R58L:Q38F:Q4F:F57H Variant of HCRBPII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	60.089, 63.849, 72.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.350 - 1.670
R-factor	0.2411
Rwork	0.239
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rct
RMSD bond length	0.006
RMSD bond angle	0.820
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.670	1.670
Rmerge	0.087	1.238
Rmeas	0.090
Number of reflections	31490	3092
<I/σ(I)>	43.84
Completeness [%]	100.0	100
Redundancy	14.6	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 4000, Sodium acetate, ammonium acetate