6WN7
Homo sapiens S100A5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 76.280, 76.280, 84.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.000 - 1.250 |
| R-factor | 0.1862 |
| Rwork | 0.171 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dir |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.167 |
| Data reduction software | MOSFLM (1.16_3549) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.980 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 151437 | 4561 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 99.8 | 94.6 |
| Redundancy | 10.6 | 8.4 |
| CC(1/2) | 1.000 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M ammonium sulfate, 20% w/v PEG8000, cryoprotectant: 25% PEG1500 |
