6WN5
1.52 Angstrom Resolution Crystal Structure of Transcriptional Regulator HdfR from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.389, 62.389, 103.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.860 - 1.520 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.20490 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.499 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.057 | 0.783 |
| Rmeas | 0.061 | 0.827 |
| Rpim | 0.019 | 0.263 |
| Number of reflections | 32133 | 1569 |
| <I/σ(I)> | 44.4 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 9.5 |
| CC(1/2) | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 292 | Protein: 7.8 mg/ml, 0.5M Sodium chloride, 0.01M Tris pH 8.3; Screen (PACT, E10): 0.2 M Sodium/Potassium phosphate, 20% (w/v) PEG 3350 |
