6WN1

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 241242

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	360.698, 43.699, 115.332
Unit cell angles	90.00, 101.69, 90.00

Refinement procedure

Resolution	43.723 - 3.150
R-factor	0.2522
Rwork	0.251
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.343	48.343	3.330
High resolution limit [Å]	3.140	9.260	3.140
Rmerge	0.150	0.027	0.889
Rmeas	0.175	0.032	1.037
Total number of observations	118945
Number of reflections	31426	1333	4779
<I/σ(I)>	8.57	34.88	1.48
Completeness [%]	98.8	97.3	94.8
Redundancy	3.785	3.475	3.705
CC(1/2)	0.993	0.999	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.4 M sodium thiocyanate, 17% (w/v) PEG 3,350