6WMK
Crystal structure of beta sheet heterodimer LHD29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.072, 38.175, 60.379 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 38.030 - 2.200 |
| R-factor | 0.2551 |
| Rwork | 0.254 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Designed Model |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.394 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc2_3793) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.220 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 12352 | 1996 |
| <I/σ(I)> | 5.2 | 1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.3 | 3 |
| CC(1/2) | 0.971 | 0.468 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Sodium Iodide, 20% PEG3350 |
