6WMG
Human Sun2 (500-717)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 79.192, 79.192, 199.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.510 - 1.900 |
| R-factor | 0.1798 |
| Rwork | 0.175 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dxt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.510 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.042 | 0.599 |
| Number of reflections | 19072 | 1319 |
| <I/σ(I)> | 24 | 1.4 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 3.7 | 3.4 |
| CC(1/2) | 0.996 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 14.5% PEG 3350, 0.2M sodium bromide, 0.1M potassium formate |
