6WMD
Human Sun2-KASH4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 80.757, 80.757, 173.909 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 69.940 - 1.500 |
R-factor | 0.1674 |
Rwork | 0.167 |
R-free | 0.18440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dxt |
RMSD bond length | 0.006 |
RMSD bond angle | 0.846 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.940 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rpim | 0.021 | |
Number of reflections | 54661 | 3726 |
<I/σ(I)> | 55.6 | |
Completeness [%] | 99.8 | 98 |
Redundancy | 20 | |
CC(1/2) | 0.999 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 17% PEG 3350, 0.2M magnesium chloride, 0.1M BisTris/HCl |