6WMB

Crystal structure of a soluble variant of full-length human APOBEC3G (pH 8.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 1 2 1
Unit cell lengths	60.238, 42.081, 194.210
Unit cell angles	90.00, 95.76, 90.00

Refinement procedure

Resolution	35.230 - 3.020
R-factor	0.2526
Rwork	0.247
R-free	0.30460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6BUX CHAIN A AND 5K81 CHAIN A
RMSD bond length	0.002
RMSD bond angle	0.419
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.210
High resolution limit [Å]	3.020	3.020
Rmerge	0.183	0.577
Number of reflections	7726	367
<I/σ(I)>	5.17	1.18
Completeness [%]	84.1	79.2
Redundancy	2.7	2.1
CC(1/2)	0.942	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.1M Tris (pH 8.0) and 5.5% w/v PEG 4000