6WM9

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 237235

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	61.900, 86.780, 90.770
Unit cell angles	91.62, 109.98, 99.88

Refinement procedure

Resolution	42.464 - 2.450
R-factor	0.2259
Rwork	0.223
R-free	0.27880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.464	42.464	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.150	0.050	0.619
Rmeas	0.179	0.059	0.736
Total number of observations	207150
Number of reflections	62389	700	4552
<I/σ(I)>	7.37	23.34	1.83
Completeness [%]	97.4	96.8	96.6
Redundancy	3.32	3.569	3.368
CC(1/2)	0.988	0.996	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M magnesium formate, 15% (w/v) PEG 3,350