6WM7

Periplasmic EDTA-binding protein EppA, orthorhombic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	84.339, 90.607, 165.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.456 - 1.560
R-factor	0.1524
Rwork	0.152
R-free	0.17070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vr5
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.570
High resolution limit [Å]	1.560	5.330	1.560
Rmerge	0.125	0.040	1.608
Rmeas	0.130	0.042	1.855
Rpim	0.035	0.011	0.895
Total number of observations	2422624
Number of reflections	179522	4858	4156
<I/σ(I)>	7.9
Completeness [%]	99.8	100	93.9
Redundancy	13.5	14.2	3.6
CC(1/2)		0.999	0.250

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	100 mM Tris, 2.0 M ammonium sulfate