6WM6

Periplasmic EDTA-binding protein EppA, tetragonal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	87.805, 87.805, 164.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.100 - 1.420
R-factor	0.1442
Rwork	0.144
R-free	0.15250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vr5
RMSD bond length	0.007
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.100	1.467
High resolution limit [Å]	1.416	1.416
Rmerge	0.160	0.860
Number of reflections	122095	11723
<I/σ(I)>	10.57	4.77
Completeness [%]	97.4
Redundancy	20
CC(1/2)	0.996	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	100 mM Tris, 2.0 M ammonium sulfate