6WLE

Crystal structure of the Zeitlupe light-state mimic G46A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-11-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9773
Spacegroup name	I 21 3
Unit cell lengths	265.822, 265.822, 265.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.590 - 3.000
R-factor	0.2041
Rwork	0.203
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5svu
RMSD bond length	0.017
RMSD bond angle	1.559
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.590	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.172
Number of reflections	62237	3079
<I/σ(I)>	17.5
Completeness [%]	99.7
Redundancy	6.4
CC(1/2)		0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M MES pH 6.0, 1.6 M Ammonium Sulfate, 0.01 M Cobalt Chloride Hexahydrate