6WKS

Structure of SARS-CoV-2 nsp16/nsp10 in complex with RNA cap analogue (m7GpppA) and S-adenosylmethionine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	168.067, 168.067, 52.304
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.002 - 1.800
Rwork	0.152
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r24
RMSD bond length	0.015
RMSD bond angle	1.851
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.002	1.867
High resolution limit [Å]	1.800	1.800
Rmerge	0.130	1.656
Rmeas	0.130	1.698
Rpim	0.042	0.374
Number of reflections	78401	7772
<I/σ(I)>	13.6	1.35
Completeness [%]	99.9	100
Redundancy	20.02	18.7
CC(1/2)	0.997	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	10% (v/v) MPD, 0.1M HEPES pH 7.0