6WKM

Fab Fragment of Anti-human LAG3 antibody (22D2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9796
Spacegroup name	P 41 21 2
Unit cell lengths	97.800, 97.800, 103.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.610 - 2.100
R-factor	0.2044
Rwork	0.203
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mim
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.650	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.072	0.300
Rmeas	0.080	0.340
Rpim	0.040	0.180
Number of reflections	29501	2378
<I/σ(I)>	16.7	4.5
Completeness [%]	99.2	98.8
Redundancy	6.5	6.4
CC(1/2)	0.980	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	PEG 8000, sodium phosphate dibasic, sodium chloride