6WK4

Fab Fragment of Anti-human LAG3 antibody (13E2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-29
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0059
Spacegroup name	C 2 2 21
Unit cell lengths	70.151, 74.048, 189.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.630 - 2.100
R-factor	0.1882
Rwork	0.186
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mim
RMSD bond length	0.007
RMSD bond angle	0.907
Data reduction software	Aimless
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.630	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.360
Rmeas	0.080	0.470
Rpim	0.050	0.230
Number of reflections	28514	2176
<I/σ(I)>	11.1	2.7
Completeness [%]	98.3	93.8
Redundancy	4.8	3.3
CC(1/2)	0.990	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	PEG 3350, potassium citrate tribasic