6WJZ

Crystal structure of human ribokinase in complex with AMPCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	46.110, 71.860, 91.430
Unit cell angles	90.00, 91.78, 90.00

Refinement procedure

Resolution	56.490 - 1.800
R-factor	0.15384
Rwork	0.152
R-free	0.18632
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c40
RMSD bond length	0.014
RMSD bond angle	1.824
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.490	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.054	0.538
Rmeas	0.060	0.605
Rpim	0.027	0.271
Number of reflections	54971	2719
<I/σ(I)>	14.5	2.3
Completeness [%]	99.2	97.7
Redundancy	4.8	4.7
CC(1/2)	0.999	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.2 M sodium acetate, 20% PEG 3350