6WJZ
Crystal structure of human ribokinase in complex with AMPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.110, 71.860, 91.430 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 56.490 - 1.800 |
| R-factor | 0.15384 |
| Rwork | 0.152 |
| R-free | 0.18632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c40 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.824 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.490 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.054 | 0.538 |
| Rmeas | 0.060 | 0.605 |
| Rpim | 0.027 | 0.271 |
| Number of reflections | 54971 | 2719 |
| <I/σ(I)> | 14.5 | 2.3 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 4.8 | 4.7 |
| CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.2 M sodium acetate, 20% PEG 3350 |
