6WJU

Fab Fragment of Anti-human LAG3 antibody (4A10)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	I 41 3 2
Unit cell lengths	258.050, 258.050, 258.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.260 - 3.100
R-factor	0.2312
Rwork	0.228
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e2t
RMSD bond length	0.004
RMSD bond angle	0.842
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.260	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.170	0.590
Rmeas	0.200	0.680
Rpim	0.090	0.330
Number of reflections	26760	4760
<I/σ(I)>	7	2.9
Completeness [%]	99.9	99.9
Redundancy	7.5	7.5
CC(1/2)	0.800	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	PEG 4000, Lithium sulphate, Tris-HCl