6WJQ

Crystal structure of WDR5 in complex with the WIN peptide of PDPK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-09
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 1 21 1
Unit cell lengths	54.518, 47.228, 118.965
Unit cell angles	90.00, 90.92, 90.00

Refinement procedure

Resolution	29.740 - 2.710
R-factor	0.2262
Rwork	0.223
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ucs
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.072	0.208
Rmeas	0.085	0.261
Rpim	0.045	0.154
Number of reflections	15751	765
<I/σ(I)>	15.11
Completeness [%]	93.2	86.2
Redundancy	2.9
CC(1/2)	0.990	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	PEG 3350, Ammonium Acetate, Bis-tris