6WIM
CdiB from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2018-04-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 112.953, 183.379, 45.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.600 |
| R-factor | 0.2486 |
| Rwork | 0.248 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wil |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 1.300 |
| Number of reflections | 29889 | 3597 |
| <I/σ(I)> | 13.9 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13 | 13.5 |
| CC(1/2) | 0.990 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 100mM Gly-Gly AMPD pH 8.5 4mM Alkalis (1mM RbCl, 1mM SrAc, 1mM CeAc,1mM BaAc) 12.5% PEG 4000 20% 1,2,6-hexanetriol |
