6WG9

Crystal structure of tetracycline destructase Tet(X7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-09-27
Detector	RDI CMOS_8M
Wavelength(s)	1.000033
Spacegroup name	P 21 21 21
Unit cell lengths	57.130, 131.970, 136.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.770 - 2.550
R-factor	0.2088
Rwork	0.207
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a6n
RMSD bond length	0.004
RMSD bond angle	0.717
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.770	2.641
High resolution limit [Å]	2.550	2.550
Rmerge	0.091	0.897
Rmeas		0.976
Rpim		0.381
Number of reflections	34444	3416
<I/σ(I)>	17.31	2.26
Completeness [%]	99.5
Redundancy	6.9
CC(1/2)	0.998	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M ammonium sulfate and 20% (w/v) PEG4000