6WF6
Streptomyces coelicolor methylmalonyl-CoA epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-04-19 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.224, 75.849, 80.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.590 - 1.390 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jc5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.842 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.390 | 1.400 |
| Rmerge | 0.165 | 1.443 |
| Rmeas | 0.178 | |
| Rpim | 0.067 | |
| Number of reflections | 58310 | 5716 |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 300 mM sodium chloride, 100 mM bis-tris:HCl pH 7.0, 26% PEG 3350, 5% PEG 400 |
