6WF1

Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H10 Hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033202
Spacegroup name	P 3 2 1
Unit cell lengths	127.021, 127.021, 158.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.580 - 4.190
R-factor	0.2656
Rwork	0.261
R-free	0.30850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tgv
RMSD bond length	0.002
RMSD bond angle	0.478
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.580	4.410
High resolution limit [Å]	4.190	4.190
Rmerge	0.110	0.581
Rmeas	0.127	0.715
Rpim	0.063	0.406
Number of reflections	9561	775
<I/σ(I)>	8.8	1.5
Completeness [%]	84.5
Redundancy	3.4
CC(1/2)	0.987	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	15% w/v PEG 4000, 0.15M Ammonium Sulfate, 0.1M MES pH 6