6WEZ
Crystal Structure of Broadly Neutralizing Antibody 3I14-D93N Mutant Bound to the Influenza A H3 Hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | .97919 |
| Spacegroup name | P 63 |
| Unit cell lengths | 130.842, 130.842, 189.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.420 - 3.210 |
| R-factor | 0.2362 |
| Rwork | 0.233 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 405I |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.674 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.420 | 3.320 |
| High resolution limit [Å] | 3.205 | 3.205 |
| Rmerge | 0.141 | 1.147 |
| Rmeas | 0.158 | 1.287 |
| Rpim | 0.072 | 0.581 |
| Number of reflections | 29818 | 2918 |
| <I/σ(I)> | 10.17 | 1.18 |
| Completeness [%] | 98.8 | 97.6 |
| Redundancy | 4.7 | 4.8 |
| CC(1/2) | 0.996 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 15% w/v PEG 4000, 0.15M Ammonium Sulfate, 0.1M MES pH 6 |
