6WEX
Crystal Structure of Broadly Neutralizing Antibody 3I14-D93N Mutant Bound to the Influenza A H6 Hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-04-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 117.810, 117.810, 438.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.260 - 3.490 |
| R-factor | 0.2814 |
| Rwork | 0.276 |
| R-free | 0.33270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xkd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.609 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.260 | 3.615 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.168 | 0.649 |
| Rmeas | 0.686 | |
| Rpim | 0.058 | 0.219 |
| Number of reflections | 15294 | 1421 |
| <I/σ(I)> | 9.89 | 3.15 |
| Completeness [%] | 99.3 | 94.5 |
| Redundancy | 9.5 | 9.6 |
| CC(1/2) | 0.995 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MIB Buffer (sodium malonate, imidazole, and boric acid in the molar ratios 2:3:3) pH 5.0, and 25% PEG 1500 |
