6WEM
Crimson 0.9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-05-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.546, 90.046, 129.518 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.040 |
| Rwork | 0.191 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ip2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.970 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.160 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 53213 | 8162 |
| <I/σ(I)> | 12.12 | 2.2 |
| Completeness [%] | 99.1 | |
| Redundancy | 7.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M MgCl2, 0.1 M Bis-Tris HCl pH 6.5, and 25% PEG 3350 |
