6WEG

Structure of Ft (MglA-SspA)-ppGpp-PigR peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.01
Spacegroup name	P 21 21 21
Unit cell lengths	65.756, 113.562, 141.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.461 - 2.950
R-factor	0.2304
Rwork	0.225
R-free	0.28980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u56
RMSD bond length	0.004
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.461	3.080
High resolution limit [Å]	2.950	2.950
Rpim	0.063	0.950
Number of reflections	22603	2028
<I/σ(I)>	7.3
Completeness [%]	98.1
Redundancy	2.9
CC(1/2)	0.997	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Peg 4000, Tris HCl pH 8