6WEA

YTH domain of human YTHDC1 with a 10mer Oligo Containing N6mA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	82.936, 42.641, 119.869
Unit cell angles	90.00, 101.79, 90.00

Refinement procedure

Resolution	40.590 - 1.800
R-factor	0.2112
Rwork	0.210
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.004
RMSD bond angle	0.682
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.590	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.134	0.889
Rpim	0.083	0.678
Number of reflections	73744	6380
<I/σ(I)>	8.7	1.1
Completeness [%]	96.9
Redundancy	3.4
CC(1/2)		0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1 M Bis-Tris pH 7.0, 0.24 M ammonium sulfate, 23% PEG 3350, 15% glycerol