6WE1

Wheat dwarf virus Rep domain complexed with a single-stranded DNA 8-mer comprising the cleavage site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	298
Detector technology	CCD
Collection date	2019-10-29
Detector	RIGAKU SATURN 944
Wavelength(s)	1.542
Spacegroup name	P 41 21 2
Unit cell lengths	50.626, 50.626, 241.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.550 - 2.612
R-factor	0.1907
Rwork	0.188
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q1m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.550	2.705
High resolution limit [Å]	2.612	2.612
Rmeas	0.112	0.609
Rpim	0.071	0.378
Number of reflections	9674	876
<I/σ(I)>	7.7	2.27
Completeness [%]	93.3	87.6
Redundancy	1.6
CC(1/2)	0.987	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	12% w/v PEG 8000, 0.1 M sodium cocadylate, pH 6.5, 0.2M zinc acetate, 25% glycerol