6WCH
Crystal structure of Laccase from Thermus thermophilus HB27 with an open conformation of beta-hairpin (Average deposited dose 7.75 MGy)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.480, 60.840, 76.100 |
| Unit cell angles | 90.00, 109.42, 90.00 |
Refinement procedure
| Resolution | 27.230 - 1.700 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu9 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (7.0.076) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.360 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.357 |
| Rmeas | 0.083 | 0.505 |
| Rpim | 0.058 | 0.357 |
| Number of reflections | 51736 | 5145 |
| <I/σ(I)> | 7.27 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1.9 | 1.8 |
| CC(1/2) | 0.995 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 277.15 | 20% PEG 4000, 20% 2-propanol, 100 mM citrate buffer |
