6WBE
Crystal structure of coiled coil region of human septin 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.82652 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.230, 60.450, 81.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.820 - 2.100 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25650 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.470 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.640 | 81.640 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
| Rmerge | 0.060 | 0.029 | 0.306 |
| Rmeas | 0.066 | 0.034 | 0.335 |
| Rpim | 0.027 | 0.016 | 0.136 |
| Total number of observations | 45377 | 606 | 3763 |
| Number of reflections | 7988 | 131 | 640 |
| <I/σ(I)> | 16.4 | 32 | 5.6 |
| Completeness [%] | 99.2 | 97.3 | 99 |
| Redundancy | 5.7 | 4.6 | 5.9 |
| CC(1/2) | 0.999 | 0.999 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M zinc acetate, 12% w/v PEG 3350 |
