6WAN

Structure of Acinetobacter baumannii Cap4 SAVED/CARF-domain containing receptor with the cyclic trinucleotide 3'3'3'-cAAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-02-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	106.498, 111.184, 163.748
Unit cell angles	90.00, 100.36, 90.00

Refinement procedure

Resolution	38.800 - 2.400
R-factor	0.2082
Rwork	0.208
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vm6
RMSD bond length	0.002
RMSD bond angle	0.605
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.800	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	145531	13668
<I/σ(I)>	8.4
Completeness [%]	99.0
Redundancy	3.6
CC(1/2)	0.996	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1 M Tris pH 8.5, 0.2 M LiSO4, 16% PEG-3350