6WAH
Crystal Structure of SmcR L139R from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-11-08 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.971, 98.998, 129.106 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.218 - 2.550 |
R-factor | 0.2315 |
Rwork | 0.228 |
R-free | 0.30310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kz9 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.856 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15_3459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.220 | 2.630 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmeas | 0.373 | 1.512 |
Rpim | 0.155 | 0.588 |
Number of reflections | 33302 | 4024 |
<I/σ(I)> | 7.7 | 1.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 6.3 |
CC(1/2) | 0.980 | 0.786 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M of Lithium Sulfate, 0.1 M Imidazole buffer pH 7.6-8 and 6-10% PEG3350 |