6WA9

Structure of the Chlamydia pneumoniae CdsV and CdsO protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	156.410, 206.610, 280.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.220 - 4.620
R-factor	0.243
Rwork	0.241
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wa6
RMSD bond length	0.003
RMSD bond angle	0.550
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX (1.18.2-3874-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.270	4.700
High resolution limit [Å]	4.620	4.620
Number of reflections	50242	4970
<I/σ(I)>	8.1
Completeness [%]	99.3
Redundancy	7.5
CC(1/2)	0.998	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294.15	100mM Bis-Tris pH 6.0, 4% w/v PEG 3350, 200mM ammonium acetate