6W9W

R80A PCNA mutant defective in BIR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-10-14
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 21 3
Unit cell lengths	122.078, 122.078, 122.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.590 - 2.650
R-factor	0.2156
Rwork	0.214
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
RMSD bond length	0.003
RMSD bond angle	0.606
Data reduction software	XDS
Data scaling software	Aimless (0.5.14)
Phasing software	pointless
Refinement software	Coot (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.595	2.720
High resolution limit [Å]	2.650	2.650
Rmerge	0.119	0.113
Rmeas	0.122
Rpim	0.026	0.487
Number of reflections	34129	2274
<I/σ(I)>	27.6	1.6
Completeness [%]	100.0	100
Redundancy	21.3	21.4
CC(1/2)	0.999	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2.2 M ammonium sulfate, 0.2 M ammonium formate