6W92

Human UHRF1 TTD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97741
Spacegroup name	P 21 21 21
Unit cell lengths	40.380, 54.870, 66.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.340 - 1.300
R-factor	0.1743
Rwork	0.174
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qqd
RMSD bond length	0.008
RMSD bond angle	0.898
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.550	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	37049	1746
<I/σ(I)>	20.9
Completeness [%]	99.8
Redundancy	6.9
CC(1/2)	0.999	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.75	295	0.1 M Bis-Tris, pH = 5.5, 25 % w/v PEG 3350