6W8E

Crystal Structure Analysis of Space-grown Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 21 2
Unit cell lengths	75.970, 75.970, 34.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.680
R-factor	0.2479
Rwork	0.244
R-free	0.30630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kxk
RMSD bond length	0.011
RMSD bond angle	1.442
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.719	53.719	2.820
High resolution limit [Å]	2.675	8.460	2.675
Rmerge		0.050	0.777
Rmeas	0.093	0.055	0.843
Rpim	0.037	0.023	0.319
Total number of observations	19338
Number of reflections	3147	129	434
<I/σ(I)>	11.7	11.3	1
Completeness [%]	99.6	98.8	99.7
Redundancy	6.1	4.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	4.6	296	PEG 6000, NaCl, Na Acetate