6W8C

K2P2.1 (TREK-1):ML335 complex, 1 mM K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	67.129, 119.783, 128.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.970 - 2.600
R-factor	0.2518
Rwork	0.251
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cq6
RMSD bond length	0.013
RMSD bond angle	1.460
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.490	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.104
Number of reflections	32731	3898
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)		0.192

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl
1	VAPOR DIFFUSION, HANGING DROP	8	277	22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl