6W8B

Structure of DNMT3A in complex with CGA DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	.5
Spacegroup name	P 32
Unit cell lengths	186.347, 186.347, 81.671
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.871 - 2.400
R-factor	0.2124
Rwork	0.212
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	123917	123895
<I/σ(I)>	10
Completeness [%]	99.9
Redundancy	5.1
CC(1/2)	0.999	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Sodium Citrate pH 4.2, 1% PEG8000