6W7T

Structure of PaP3 small terminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	108.302, 121.152, 129.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.010
R-factor	0.2521
Rwork	0.251
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zqp
RMSD bond length	0.005
RMSD bond angle	0.953
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rpim	0.035	0.696
Number of reflections	32945	3308
<I/σ(I)>	49.1	1.7
Completeness [%]	98.4	99.6
Redundancy	6.4	6.4
CC(1/2)		0.398

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM KCl, 25 mM MgCl2, 50 mM Na-Cacodylate trihydrate pH 6.0, 15% (v/v) 2-propanol