6W6X
Crystal Structure of ABLE Apo-protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.426, 43.648, 91.835 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 45.905 - 1.297 |
| R-factor | 0.1688 |
| Rwork | 0.166 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Designed model |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.905 | 45.905 | 1.340 |
| High resolution limit [Å] | 1.267 | 4.010 | 1.270 |
| Rmerge | 0.045 | 0.507 | |
| Rmeas | 0.073 | 0.051 | 0.641 |
| Rpim | 0.028 | 0.019 | 0.266 |
| Total number of observations | 330471 | 12298 | 39326 |
| Number of reflections | 53412 | 1797 | 7501 |
| <I/σ(I)> | 12.4 | 32.8 | 1.6 |
| Completeness [%] | 98.5 | 99.9 | 95.2 |
| Redundancy | 6.2 | 6.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.6 M AmSO4, 0.1 M Na acetate |
