6W67
The structure of S172A Keap1-BTB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 42.680, 42.680, 266.236 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.370 - 2.200 |
| R-factor | 0.2412 |
| Rwork | 0.238 |
| R-free | 0.30530 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4cxi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.853 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.373 | 44.370 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.198 | 0.033 | 3.835 |
| Rmeas | 0.202 | 0.034 | 3.910 |
| Rpim | 0.040 | 0.009 | 0.755 |
| Total number of observations | 2845 | 17870 | |
| Number of reflections | 8245 | 185 | 677 |
| <I/σ(I)> | 16.1 | 67.1 | 1.1 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 24.5 | 15.4 | 26.4 |
| CC(1/2) | 1.000 | 1.000 | 0.713 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 160-400 mM lithium acetate and 14-18% PEG 3350 1:1 with protein at 11mg/ml in 150 mM NaCl, 25 mM Tris-HCl pH 8.0, 1 mM TCEP |
