6W5Q
Structure of the globular C-terminal domain of P. aeruginosa LpoP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.757, 154.584, 54.083 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 48.757 - 2.200 |
| R-factor | 0.1955 |
| Rwork | 0.194 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model based on 5XW7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.760 | 48.760 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.057 | 0.025 | 0.378 |
| Rmeas | 0.069 | 0.030 | 0.453 |
| Rpim | 0.037 | 0.016 | 0.247 |
| Total number of observations | 129787 | 1774 | 11062 |
| Number of reflections | 39974 | 567 | 3454 |
| <I/σ(I)> | 12.7 | 28.6 | 3.2 |
| Completeness [%] | 98.7 | 95.4 | 98.6 |
| Redundancy | 3.2 | 3.1 | 3.2 |
| CC(1/2) | 0.998 | 0.999 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | (30 mg/mL purified protein in 20 mM HEPES, pH 8.0, 150 mM NaCl) and 1 uL of mother liquor (0.1 M HEPES pH 7.5, 2.5 M ammonium sulfate, 1% PEG 400, 2.5% 1,2-butanediol) |
