6W5D
Crystal Structure of Fab RSB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.360, 82.487, 75.965 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 35.550 - 2.000 |
| R-factor | 0.2339 |
| Rwork | 0.232 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6w52 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.550 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 55626 | 4080 |
| <I/σ(I)> | 14.66 | |
| Completeness [%] | 89.1 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.840 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M ammonium sulfate, 0.1 M bis-tris pH 5.5, 25% PEG 3350 |
