6W43
APE1 AP-endonuclease product complex R237C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-22 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.429, 61.418, 72.121 |
| Unit cell angles | 83.75, 79.03, 88.32 |
Refinement procedure
| Resolution | 24.910 - 1.990 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.991 |
| Data reduction software | PHENIX (1.17.1_3660) |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmeas | 0.107 | 0.599 |
| Number of reflections | 91241 | 3744 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% PEG20000, 100 mM sodium citrate, pH 5.0, and 200 mM MgCl2 |
