6W3N

APE1 exonuclease substrate complex D148E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-07
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	71.554, 65.074, 91.424
Unit cell angles	90.00, 109.93, 90.00

Refinement procedure

Resolution	24.760 - 2.690
R-factor	0.2549
Rwork	0.249
R-free	0.30840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w4n
RMSD bond length	0.012
RMSD bond angle	1.359
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.750
High resolution limit [Å]	2.690	2.700
Rmeas	0.210	1.000
Number of reflections	37101	1645
<I/σ(I)>	7.8
Completeness [%]	96.2
Redundancy	2.9
CC(1/2)		0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	7% PEG20000, 100 mM sodium citrate, 15% glycerol, 5 mM calcium chloride