6W37
STRUCTURE OF THE SARS-CoV-2 ORF7A ENCODED ACCESSORY PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.393, 57.393, 55.918 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.150 - 2.900 |
| R-factor | 0.2551 |
| Rwork | 0.236 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xak |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.413 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.150 | 37.140 | 3.080 |
| High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
| Rmerge | 0.249 | 0.088 | 1.042 |
| Rmeas | 0.263 | 0.095 | 1.096 |
| Rpim | 0.083 | 0.034 | 0.335 |
| Total number of observations | 846 | 4257 | |
| Number of reflections | 2553 | 116 | 399 |
| <I/σ(I)> | 9.8 | 19.9 | 2.6 |
| Completeness [%] | 99.9 | 98.6 | 100 |
| Redundancy | 9.9 | 7.3 | 10.7 |
| CC(1/2) | 0.992 | 0.992 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 20% PEG 4000, 0.2 M CaCl2, 0.1M Tris-HCl pH8.5 |
